In addition to individual research laboratory spaces for each faculty/student research team to conduct their work, the Chemistry Department offers students research-grade instrumentation to support their education. The Neidig-Garber Science Center is a PUI facility and our instrumentation is impressive by many standards. In addition to their research application, we incorporate all instruments in the chemistry curriculum, beginning as soon as the freshman year, and through our many funded faculty/student summer research projects.
Buck Scientific 205 Atomic Absorption Spectrophotometer (AA) System. This instruments is used for the analysis of metal ions in aqueous solution. The instrument aspirates the solution into a flame where the absorbance of the metal is measured bu ultraviolet or visible light. Used often for environmental stream water analysis of heavy metal pollutants.
Anton Paar Monowave 50 Reactor. The modern and compact synthesis reactor is suitable for performing fast and green chemistry in educational and research labs. The high pressure/temperature reactor is appropriate for condensation reactions, nucleophilic substitutions, cycloadditions, metal-catalyzed coupling reactions, ionic liquid syntheses, and Grignard reactions.
Photon Technology International QuantaMaster, Model QM-40 (QM-400) Spectrofluorometer with LEDStrobe Fluorescence Lifetimes Enhancement. Fluorescence spectroscopy is a technique used to analyze compounds that emit light of lower energy after being excited by higher energy light. The Lifetimes Enhancement determines the time a fluorophore spends in the excited state before emitting a photon and returning to the ground state.
Thermo Electron Nicolet IS5 MID-IR System with Optional ID7 ATR Accessory. This instrument measures the absorbance of infrared radiation by organic and inorganic compounds. The infrared spectrum taken by this instrument can serve as a "fingerprint" for a molecule. It is used in chemical research, forensic, and environmental chemistry. The ATR spectroscopy works especially well for opaque samples or samples that are too thick for transmission measurements. The technique gives information about the surface properties and condition of materials and can be useful for both qualitative and quantitative measurements.
SpectraMax® M3 Multi-Mode Microplate Reader System. The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiplate readers are used to quantify protein, gene expression and various metabolic processes.
T.A. Instruments Q20 Differential Scanning Calorimeter with Mass Flow Control (DSC) System. The differential scanning calorimeter takes sensitive measurements of heat flow into and out of samples. The DSC is outfitted with an RCS90 cooling system, enabling a temperature range of -90 to 400 deg C. It is used for detecting thermal transtions in polymers, measuring heats of reaction, determining melting/crystallization temperatures, and investigating the kinetics of chemical and physical processes.
Waters Alliance HPLC-Quattro Micro Mass Spectrometer (LC-MS-MS) System. This instrument combines high performance liquid chromatography with electrospray mass spectrometry. The instrument is used to identify and quantitate trace levels of metabolites in complex cellular extracts and to determine pollutants in dilute wastestreams.
Bruker Avance 300 MHz Fourier-Transform Superconducting Broadband Nuclear Magnetic Resonance Spectrometer (FT-NMR). This instrument allows our students to determine the chemical structures of organic molecules, even very complex ones. FTNMR is the key instrument in research areas related to organic chemistry.
Bruker MicroFlex Reflectron MALDI-TOF Mass Spectrometer. This instrument, based on matrix-assisted laser desorption ionization (MALDI), is used to determine the molecular massess of intermediate to very large sized molecules. Our students use it for studying proteins, peptides, nucleic acids, inorganic complexes, and organic polymers.
Agilent 6898/5973 Gas Chromatograph-Mass Spectrometer (GC-MS). This instrument combines the powerful separating ability of capillary gas chromatography with quadrupole mass spectrometry to create an instrument that can separate the components of complex mixtures (like gasoline or perfume) and identify each of the many components found in those mixtures. This instrument is a workhorse in forensic and environmental chemistry.
Nicolet 380 Fourier-Transform Infrared Spectrometer (FTIR). This instrument measures the absorbance of infrared radiation by organic and inorganic compounds. The infrared spectrum taken by this instrument can serve as a "fingerprint" for a molecule. It is used in chemical research, forensic, and environmental chemistry.
ThermoElectron Evolution 300 Rapid Scanning Double Beam Ultraviolet-Visible Spectrophotometer with Peltier Temperature Control (UV-VIS). This spectrometer measures absorbance of light in the ultraviolet and visible ranges of the electromagnetic spectrum. It is used commonly for studying kinetics, including enzyme assays, for determining equilibrium constants, and for determining the amounts of compounds in solution.
Teledyne Isco CombiFlash RF-200 Automated Flash Chromatography System. This new preparative liquid chromatograph is a workhorse in the laboratories of Dr. Harris and Peelen. Its primary use is to purify and collect products resulting from organic synthesis.
Agilent Series 1100 Quaternary High-Performance Liquid Chromatograph with Autosampler (HPLC). This instrument is used by our students to separate, identify, and quantitate components of complex mixtures, often in aqueous solution. Used in all areas of chemistry and in biological applications.
Molecular Devices SPECTRAmax PLUS Microplate Spectrophotometer (UV-VIS). This instrument allows students in biochemistry to initiate a large number of enzyme assays in the wells of a micro plate, and the instrument subsequently follows all of the assays automatically.
MBraun LabMaster 130 Inert Atmosphere Chamber (Glove Box). The glove box allows students to work in a chamber to prepare solutions of compounds that cannot be exposed to water or oxygen. The atmosphere within the chamber is entirely nitrogen gas which is inert and unreactive. Students work in the box by placing their arms into long gloves that reach into the chamber.
Princeton Applied Research Model 273A Voltammetry/Coulometry Instrumentation (CV). This instrument uses two- and three-electrode arrangements to carry out a variety of electrochemical techniques. We use it most often for cyclic voltammetry to determine standard reduction potentials for compounds that are readily oxidized or reduced.